UC Flow Cytometry Core

College of Medicine Research Core Facilities

The UC College of Medicine houses several research core facilities designated as core service centers. These facilities exist within multiple departments but are collectively supported by the College of Medicine Office of Research through the Associate Dean for Research Core Facilities: Ken Greis, PhD. (ken.greis@uc.edu; Tel: 513-558-7102).

The service center designation signifies the rates charged by each of these facilities have been reviewed and approved by the UC Government Cost Compliance Office; thus, the service fees can be charged to federal grants and contracts. Details related to the services offered and the internal rates for each of the cores are provided below. Since these rates are substantially subsidized by the University, external investigators should contact individual core directors to get a rate quote.

Resources to offset some of the cost of the core services may be available through a variety of centers and institutes across UC depending on an investigator’s affiliation. Information for support from the CCTST is provided here:

Center for Clinical and Translational Science and Training (CCTST) Just-in-Time Core Support

UC invesitgators also hvae full access to shared resource cores at Cincinnati Children's Hospital. Details are provided here:

Shared Facilities | Cincinnati Children's (cincinnatichildrens.org)

We have recently transitioned our core facilities booking and management to the PPMS system from Stratocore. To book and access services from the core facilities, please log in or create an account in Stratocore via:

My PPMS Dashboard

Stratocore Account Creation Guides:

New UC PI Account Creation

New UC User Account Creation

New External User Account Creation

UC Flow Cytometry Core

The Flow Core supports a Beckman CytoFLEX S with 9 detectors and 4 lasers in CARE 2874 and a FACSCalibur 4 color flow cytometer system in CARE 2876. Access is available 24 hrs a day and 7 days per week once investigators are registered in Stratocore and approved by the core director.

To book equipment and/or access services from the Flow Core, please login or create an account in Stratocore at https://ppms.us/uc/start/.

For access to the instruments and the online calendar, please contact Dr. Miller.

Grant Information

A general NIH description of facilities and equipment for this core may be accessed with this link - UC Flow Cytometry Core NIH Summary May 2024; however, it is highly recommended that you discuss your specific core needs with the core director or manager while preparing the grant application since they can likely provide tailored information regarding their capabilities to enhance your application.

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Services/Internal Fees

Service	Cost
CytoFLEX S System Access (4 laser, 9 color)	$20 per half hour
FACSCalibur System access (2 Laser, 4 color)	$20 per half hour

Equipment

FACSCalibur Four Color Flow Cytometer

The FACSCalibur is configured as follows:

FL1 Channel - Blue Laser (488nm) and 515-545nm Emission Filter (FITC, GFP, etc.)
FL2 Channel - Blue Laser (488nm) and 564-601nm Emission Filter (PE, PI, RFP, etc.)
FL3 Channel - Blue Laser (488nm) and 670LP Emission Filter (7-AAD, PerCP, etc)
FL4 Channel - Red Laser (635nm) and 653-669nm Emission Filter (APC, Cy5, etc.)

It is running the BS CellQuest Pro Software.

Beckman CytoFLEX S V2-B2-Y3-R2 Flow Cytometer (9 Detectors, 4 Lasers)

Here are the channels currently configured (in bold) the ones in italics can be utilized by repositioning the filters, which is relatively easy to do.

CytoFLEX (4 laser, 9 color)

Violet Laser (405nm)                               Emmision Filter
PB450 (PacBlue, eFluor450)                           450/45
KO525 (Krome Orange, Zombie Aqua)           525/40
BV605                                                              610/20
BV650                                                              660/20
BV786                                                              780/60

Blue Laser (488nm)
FITC (GFP, Alexa488, Venus)                           525/40
PerCP (PC5, PC5.5)                                         690/50

Yellow/Green Laser (561nm)
PE (PI, Sytox Orange, DsRed, TdTomato) 585/42
ECD (mCherry, Texas Red)                              610/20
PC7 780/60
PC5 690/50

Red Laser (638nm)
APC (AlexaFluor 647, eFluor660)                    660/10
APC-A750 (APC-Cy7, APC eFluor780             780/60
APC-A700 (AlexaFluor 700)