Terry L. Kirley, PhD

Kirley, Terence L; Norman, Andrew B 2023. Reformulation and Thermal Stability of a Therapeutic Anti-Cocaine mAb. Journal of pharmaceutical sciences, ,

Kirley, Terence L; Norman, Andrew B 2023. Characterization and optimization of fluorescein isothiocyanate labeling of humanized h2E2 anti-cocaine mAb. Biochemistry and biophysics reports, 35 , 101520

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B 2022. Tyrosine nitration of a humanized anti-cocaine mAb differentially affects ligand binding of cocaine and its metabolites. Biochemistry and biophysics reports, 30 , 101278

Kirley, Terence L; Norman, Andrew B 2022. Critical evaluation of fluorescent dyes to evaluate the stability and ligand binding properties of an anti-cocaine mAb, h2E2. Journal of immunological methods, , 113323

Kirley, Terence L; Norman, Andrew B 2022. Isothermal titration calorimetry determination of thermodynamics of binding of cocaine and its metabolites to humanized h2E2 anti-cocaine mAb. Biochemistry and biophysics reports, 32 , 101354

Kirley, Terence L; Norman, Andrew B; Greis, Kenneth D 2022. Oxidation of specific tryptophan residues inhibits high-affinity binding of cocaine and its metabolites to a humanized anticocaine mAb. The Journal of biological chemistry, 298 3, 101689

Kirley, Terence L; Norman, Andrew B 2021. Ligand binding to a humanized anti-cocaine mAb measured by dye absorption spectroscopy. Biochemical and biophysical research communications, 535 , 93-98

Kirley, Terence L; Norman, Andrew B 2021. Cocaine binding to the Fab fragment of a humanized anti-cocaine mAb quantitated by dye absorption and fluorescence spectroscopy. Journal of immunological methods, 496 , 113103

Gallagher, Zahra J; Fleetwood, Sara; Kirley, Terence L; Shaw, Maureen A; Mullins, Eric S; Ayres, Neil; Foster, E Johan 2020. Heparin Mimic Material Derived from Cellulose Nanocrystals. Biomacromolecules, 21 3, 1103-1111

Kirley, Terence L; Norman, Andrew B 2020. Ligand binding to a humanized anti-cocaine mAb detected by non-reducing SDS-PAGE. Biochemistry and biophysics reports, 23 , 100795

Kirley, Terence L; Norman, Andrew B 2020. Multi-domain unfolding of the Fab fragment of a humanized anti-cocaine mAb characterized by non-reducing SDS-PAGE. Biochemical and biophysical research communications, 533 3, 580-585

Kirley, Terence L; Norman, Andrew B; Wetzel, Hanna N 2020. A novel differential scanning fluorimetry analysis of a humanized anti-cocaine mAb and its ligand binding characteristics. Journal of immunological methods, 476 , 112676

Tan, Kemin; Zhou, Min; Ahrendt, Angela J; Duke, Norma E C; Tabaja, Nassif; Ball, William J; Kirley, Terence L; Norman, Andrew B; Joachimiak, Andrzej; Schiffer, Marianne; Wilton, Rosemarie; Pokkuluri, P Raj 2019. Structural analysis of free and liganded forms of the Fab fragment of a high-affinity anti-cocaine antibody, h2E2. Acta crystallographica. Section F, Structural biology communications, 75 Pt 11, 697-706

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B 2018. Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE. Biochemistry and biophysics reports, 16 , 138-144

Kirley, Terence L; Norman, Andrew B 2018. Unfolding of IgG domains detected by non-reducing SDS-PAGE. Biochemical and biophysical research communications, 503 2, 944-949

Kirley, Terence L; Norman, Andrew B 2018. Unfolding of IgG domains detected by non-reducing SDS-PAGE. Biochemical and biophysical research communications, 503 2, 944-949

Webster, Rose P; Cohen, Cinder F; Saeed, Fatima O; Wetzel, Hanna N; Ball, William J; Kirley, Terence L; Norman, Andrew B 2018. Evaluation of methods to reduce background using the Python-based ELISA_QC program. Journal of immunological methods, ,

Q. Chai, Y. Huang, T. L. Kirley and N. Ayres 2017. Shape memory polymer foams prepared from a heparin-inspired polyurethane/urea Polymer Chemistry, 8 , 5039-5048

Wetzel, Hanna N; Webster, Rose P; Saeed, Fatima O; Kirley, Terence L; Ball, William J; Norman, Andrew B 2017. Characterization of a recombinant humanized anti-cocaine monoclonal antibody produced from multiple clones for the selection of a master cell bank candidate. Biochemical and biophysical research communications, 487 3, 690-694

Contreras, Christopher J; Segvich, Dyann M; Mahalingan, Krishna; Chikwana, Vimbai M; Kirley, Terence L; Hurley, Thomas D; DePaoli-Roach, Anna A; Roach, Peter J 2016. Incorporation of phosphate into glycogen by glycogen synthase. Archives of biochemistry and biophysics, 597 , 21-9

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B 2016. Selective disulfide reduction for labeling and enhancement of Fab antibody fragments. Biochemical and biophysical research communications, 480 4, 752-757

Kirley, Terence L; Greis, Kenneth D; Norman, Andrew B 2016. Structural characterization of expressed monoclonal antibodies by single sample mass spectral analysis after IdeS proteolysis. Biochemical and biophysical research communications, 477 3, 363-8

Kirley, Terence L; Norman, Andrew B 2015. Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragment. Human vaccines & immunotherapeutics, 11 2, 458-67

Kirley, Terence L; Norman, Andrew B 2015. Characterization of a recombinant humanized anti-cocaine monoclonal antibody and its Fab fragment. Human vaccines & immunotherapeutics, 11 2, 458-67

Huang, Yongshun; Shaw, Maureen A; Mullins, Eric S; Kirley, Terence L; Ayres, Neil 2014. Synthesis and anticoagulant activity of polyureas containing sulfated carbohydrates. Biomacromolecules, 15 12, 4455-66

S.U. Argekar, T.L. Kirley, D.W. Schaefer 2013. Determination of Structure-Property Relationships for 3-aminopropyltriethoxysilane (APTES) Films Using X-Ray Reflectivity Journal of Materials Research, 28 8, 1118-1128

Gaddie, Keith J; Kirley, Terence L 2010. Proline residues link the active site to transmembrane domain movements in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3). Purinergic signalling, 6 3, 327-37

Ivanenkov, Vasily V; Crawford, Patrick A; Toyama, Aimi; Sévigny, Jean; Kirley, Terence L 2010. Epitope mapping in cell surface proteins by site-directed masking: defining the structural elements of NTPDase3 inhibition by a monoclonal antibody. Protein engineering, design & selection : PEDS, 23 7, 579-88

Gaddie, Keith J; Kirley, Terence L 2009. Conserved polar residues stabilize transmembrane domains and promote oligomerization in human nucleoside triphosphate diphosphohydrolase 3. Biochemistry, 48 40, 9437-47

Munkonda, Mercedes N; Pelletier, Julie; Ivanenkov, Vasily V; Fausther, Michel; Tremblay, Alain; Künzli, Beat; Kirley, Terence L; Sévigny, Jean 2009. Characterization of a monoclonal antibody as the first specific inhibitor of human NTP diphosphohydrolase-3 : partial characterization of the inhibitory epitope and potential applications. The FEBS journal, 276 2, 479-96

Ivanenkov, Vasily V; Sévigny, Jean; Kirley, Terence L 2008. Trafficking and intracellular ATPase activity of human ecto-nucleotidase NTPDase3 and the effect of ER-targeted NTPDase3 on protein folding. Biochemistry, 47 35, 9184-97

Mingyan Yang, Katsunori Horii, Andrew B. Herr, and Terence L. Kirley 2008. Characterization and importance of the dimer interface of human calcium-activated nucleotidase Biochemistry, 47 , 771-778

Yang, Mingyan; Horii, Katsunori; Herr, Andrew B; Kirley, Terence L 2008. Characterization and importance of the dimer interface of human calcium-activated nucleotidase. Biochemistry, 47 2, 771-8

Yang, Mingyan; Kirley, Terence L 2008. Engineered human soluble calcium-activated nucleotidase inhibits coagulation in vitro and thrombosis in vivo. Thrombosis research, 122 4, 541-8

Crawford, Patrick A; Gaddie, Keith J; Smith, Thomas M; Kirley, Terence L 2007. Characterization of an alternative splice variant of human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): a possible modulator of nucleotidase activity and purinergic signaling. Archives of biochemistry and biophysics, 457 1, 7-15

Kittel, Agnes; Sperlágh, Beata; Pelletier, Julie; Sévigny, Jean; Kirley, Terence L 2007. Transient changes in the localization and activity of ecto-nucleotidases in rat hippocampus following lipopolysaccharide treatment. International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 25 5, 275-82

Belcher, S M; Zsarnovszky, A; Crawford, P A; Hemani, H; Spurling, L; Kirley, T L 2006. Immunolocalization of ecto-nucleoside triphosphate diphosphohydrolase 3 in rat brain: implications for modulation of multiple homeostatic systems including feeding and sleep-wake behaviors. Neuroscience, 137 4, 1331-46

Kirley, Terence L; Crawford, Patrick A; Smith, Thomas M 2006. The structure of the nucleoside triphosphate diphosphohydrolases (NTPDases) as revealed by mutagenic and computational modeling analyses. Purinergic signalling, 2 2, 379-89

M. Yang, K. Horii, A.B. Herr, and T.L. Kirley 2006. Calcium-dependent dimerization of human soluble calcium activated nucleotidase: characterization of the dimer interface J. Biol. Chem., 281 , 28307-28317

Smith, Thomas M; Kirley, Terence L 2006. The calcium activated nucleotidases: A diverse family of soluble and membrane associated nucleotide hydrolyzing enzymes. Purinergic signalling, 2 2, 327-33

T.L. Kirley 2006. Nucleoside triphosphate diphosphohydrolase-3 (NTPDase3) AfCS-Nature Molecule Pages, ,

T.L. Kirley, P.A. Crawford, and T.M. Smith 2006. The structure of the nucleoside triphosphate diphosphohydrolases (NTPDases) as revealed by mutagenic and computational modeling analyses Purinergic Signalling, 2 , 379-389

T.M. Smith and T.L. Kirley 2006. The calcium activated nucleotidases: a diverse family of soluble and membrane associated nucleotide hydrolyzing enzymes Purinergic Signalling, 2 , 327-333

Yang, Mingyan; Horii, Katsunori; Herr, Andrew B; Kirley, Terence L 2006. Calcium-dependent dimerization of human soluble calcium activated nucleotidase: characterization of the dimer interface. The Journal of biological chemistry, 281 38, 28307-17

Basu, Saswata; Kirley, Terence L 2005. Identification of a tyrosine residue responsible for N-acetylimidazole-induced increase of activity of ecto-nucleoside triphosphate diphosphohydrolase 3. Purinergic signalling, 1 3, 271-80

D.M. Murphy-Piedmonte, P.A. Crawford and T.L. Kirley 2005. Bacterial Expression, Folding, Purification and Characterization of Soluble NTPDase5 (CD39L4) Ecto-nucleotidase Biochim. Biophys. Acta., 1747 , 251-259

F. Kukulski, S. A. Lévesque, É. G. Lavoie, J. Lecka, F. Bigonnesse, A. F. Knowles, S. C. Robson, T. L. Kirley and J. Sévigny 2005. Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8 Purinergic Signalling, 1 , 193-204

Ivanenkov, Vasily V; Meller, Jarek; Kirley, Terence L 2005. Characterization of disulfide bonds in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3): implications for NTPDase structural modeling. Biochemistry, 44 25, 8998-9012

Kukulski, F; Lévesque, S A; Lavoie, E G; Lecka, J; Bigonnesse, F; Knowles, A F; Robson, S C; Kirley, T L; Sévigny, J 2005. Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8. Purinergic signalling, 1 2, 193-204

Murphy-Piedmonte, Deirdre M; Crawford, Patrick A; Kirley, Terence L 2005. Bacterial expression, folding, purification and characterization of soluble NTPDase5 (CD39L4) ecto-nucleotidase. Biochimica et biophysica acta, 1747 2, 251-9

V.V. Ivanenkov, J. Meller and T.L. Kirley 2005. Characterization of Disulfide Bonds in Human Nucleoside Triphosphate Diphosphohydrolase 3 (NTPDase3): Implications for NTPDase Structural Modeling Biochemistry, 44 , 8998-9012

Basu, Saswata; Murphy-Piedmonte, Deirdre M; Kirley, Terence L 2004. Conserved lysine 79 is important for activity of ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3). Purinergic signalling, 1 1, 51-8

Yang, Mingyan; Kirley, Terence L 2004. Site-directed mutagenesis of human soluble calcium-activated nucleotidase 1 (hSCAN-1): identification of residues essential for enzyme activity and the Ca(2+)-induced conformational change. Biochemistry, 43 28, 9185-94

D.M. Murphy and T.L. Kirley 2003. Asparagine 81, An Invariant Glycosylation Site Near Apyrase Conserved Region 1, is Essential for Full Enzymatic Activity of Ecto Nucleoside Triphosphate Diphosphohydrolase 3 Arch. Biochem. Biophys., 413 , 107-115

D.M. Murphy, V.V. Ivanenkov and T.L. Kirley 2003. Bacterial expression and characterization of a novel, soluble, calcium binding and calcium activated human nucleotidase Biochemistry, 42 , 2412-2421

Ivanenkov, Vasily V; Murphy-Piedmonte, Deirdre M; Kirley, Terence L 2003. Bacterial expression, characterization, and disulfide bond determination of soluble human NTPDase6 (CD39L2) nucleotidase: implications for structure and function. Biochemistry, 42 40, 11726-35

Murphy, Deirdre M; Ivanenkov, Vasily V; Kirley, Terence L 2003. Bacterial expression and characterization of a novel, soluble, calcium-binding, and calcium-activated human nucleotidase. Biochemistry, 42 8, 2412-21

Murphy, Deirdre M; Kirley, Terence L 2003. Asparagine 81, an invariant glycosylation site near apyrase conserved region 1, is essential for full enzymatic activity of ecto-nucleoside triphosphate diphosphohydrolase 3. Archives of biochemistry and biophysics, 413 1, 107-15

E. Wood, M.J. Broekman, T.L. Kirley, S. Diani-Moore, M. Tickner, J.H.F. Drosopoulos, N. Islam, J.I. Park, A.J. Marcus and A.B. Rifkind 2002. Cell-type specificity of ectonucleotidase expression and upregulation by 2,3,7,8-tetrachlorodibenzo-p-dioxin Arch. Biochem. Biophys., 407 , 49-62

Murphy, Deirdre M; Ivanenkov, Vasily V; Kirley, Terence L 2002. Identification of cysteine residues responsible for oxidative cross-linking and chemical inhibition of human nucleoside-triphosphate diphosphohydrolase 3. The Journal of biological chemistry, 277 8, 6162-9

Smith, Thomas M; Hicks-Berger, Carrie A; Kim, Sunkyu; Kirley, Terence L 2002. Cloning, expression, and characterization of a soluble calcium-activated nucleotidase, a human enzyme belonging to a new family of extracellular nucleotidases. Archives of biochemistry and biophysics, 406 1, 105-15

Wood, Emily; Broekman, M Johan; Kirley, Terence L; Diani-Moore, Silvia; Tickner, Michelle; Drosopoulos, Joan H F; Islam, Naziba; Park, Joshua I; Marcus, Aaron J; Rifkind, Arleen B 2002. Cell-type specificity of ectonucleotidase expression and upregulation by 2,3,7,8-tetrachlorodibenzo-p-dioxin. Archives of biochemistry and biophysics, 407 1, 49-62

Hicks-Berger, C A; Yang, F; Smith, T M; Kirley, T L 2001. The importance of histidine residues in human ecto-nucleoside triphosphate diphosphohydrolase-3 as determined by site-directed mutagenesis. Biochimica et biophysica acta, 1547 1, 72-81

Kirley, T L; Yang, F; Ivanenkov, V V 2001. Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of conserved glycine residues and the identification of additional conserved protein motifs in eNTPDases. Archives of biochemistry and biophysics, 395 1, 94-102

Yang, F; Hicks-Berger, C A; Smith, T M; Kirley, T L 2001. Site-directed mutagenesis of human nucleoside triphosphate diphosphohydrolase 3: the importance of residues in the apyrase conserved regions. Biochemistry, 40 13, 3943-50

Hicks-Berger, C A; Chadwick, B P; Frischauf, A M; Kirley, T L 2000. Expression and characterization of soluble and membrane-bound human nucleoside triphosphate diphosphohydrolase 6 (CD39L2). The Journal of biological chemistry, 275 44, 34041-5

Hicks-Berger, C A; Kirley, T L 2000. Expression and characterization of human ecto-ATPase and chimeras with CD39 ecto-apyrase. IUBMB life, 50 1, 43-50

Kirley, T L; Gerber, L K; Smith, T M 1999. Expression and characterization of chicken muscle ecto-ATPase in mammalian COS cells. IUBMB life, 48 1, 67-72

Smith, T M; Kirley, T L 1999. Glycosylation is essential for functional expression of a human brain ecto-apyrase. Biochemistry, 38 5, 1509-16

Smith, T M; Kirley, T L 1999. Site-directed mutagenesis of a human brain ecto-apyrase: evidence that the E-type ATPases are related to the actin/heat shock 70/sugar kinase superfamily. Biochemistry, 38 1, 321-8

Smith, T M; Lewis Carl, S A; Kirley, T L 1999. Mutagenesis of two conserved tryptophan residues of the E-type ATPases: inactivation and conversion of an ecto-apyrase to an ecto-NTPase. Biochemistry, 38 18, 5849-57

Lewis Carl, S A; Smith, T M; Kirley, T L 1998. Cross-linking induces homodimer formation and inhibits enzymatic activity of chicken stomach ecto-apyrase. Biochemistry and molecular biology international, 44 3, 463-70

Smith, T M; Carl, S A; Kirley, T L 1998. Immunological detection of ecto-ATPase in chicken and rat tissues: characterization, distribution, and a cautionary note. Biochemistry and molecular biology international, 45 5, 1057-66

Smith, T M; Kirley, T L 1998. Cloning, sequencing, and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases1. Biochimica et biophysica acta, 1386 1, 65-78

T.M. Smith and T.L. Kirley 1998. Cloning, sequencing and expression of a human brain ecto-apyrase related to both the ecto-ATPases and CD39 ecto-apyrases Biochem. Biophys. Acta, 1386 , 65-78

Dombrowski, K E; Brewer, K A; Maleckar, J R; Kirley, T L; Thomas, J W; Kapp, J A 1997. Identification and partial characterization of ectoATPase expressed by immortalized B lymphocytes. Archives of biochemistry and biophysics, 340 1, 10-8

Kirley, T L 1997. Complementary DNA cloning and sequencing of the chicken muscle ecto-ATPase. Homology with the lymphoid cell activation antigen CD39. The Journal of biological chemistry, 272 2, 1076-81

Lewis-Carl, S; Kirley, T L 1997. Immunolocalization of the ecto-ATPase and ecto-apyrase in chicken gizzard and stomach. Purification and N-terminal sequence of the stomach ecto-apyrase. The Journal of biological chemistry, 272 38, 23645-52

Smith, T M; Kirley, T L; Hennessey, T M 1997. A soluble ecto-ATPase from Tetrahymena thermophila: purification and similarity to the membrane-bound ecto-ATPase of smooth muscle. Archives of biochemistry and biophysics, 337 2, 351-9

Stout, J G; Kirley, T L 1996. Control of cell membrane ecto-ATPase by oligomerization state: intermolecular cross-linking modulates ATPase activity. Biochemistry, 35 25, 8289-98

Stout, J G; Kirley, T L 1995. Inhibition of purified chicken gizzard smooth muscle ecto-ATPAse by P2 purinoceptor antagonists. Biochemistry and molecular biology international, 36 5, 927-34

Stout, J G; Strobel, R S; Kirley, T L 1995. Identification and immunolocalization of ecto-ATPDase in chicken stomach. Biochemistry and molecular biology international, 36 3, 529-35

Stout, J G; Strobel, R S; Kirley, T L 1995. Properties of and proteins associated with the extracellular ATPase of chicken gizzard smooth muscle. A monoclonal antibody study. The Journal of biological chemistry, 270 20, 11845-50

Peng, M; Fan, H; Kirley, T L; Caswell, A H; Schwartz, A 1994. Structural diversity of triadin in skeletal muscle and evidence of its existence in heart. FEBS letters, 348 1, 17-20

Stout, J G; Brittsan, A; Kirley, T L 1994. Brain ECTO-Mg-ATPase is not the neural cell adhesion molecule. Biochemistry and molecular biology international, 33 6, 1091-8

Stout, J G; Kirley, T L 1994. Purification and characterization of the ecto-Mg-ATPase of chicken gizzard smooth muscle. Journal of biochemical and biophysical methods, 29 1, 61-75

Stout, J G; Kirley, T L 1994. Tissue distribution of ecto-Mg-ATPase in adult and embryonic chicken. Biochemistry and molecular biology international, 32 4, 745-53

Treuheit, M J; Ataei, A; Wallick, E T; Kirley, T L 1993. Purification of the alpha and beta subunits of (Na,K)-ATPase by continuous elution electrophoresis. Preparative biochemistry, 23 3, 375-87

Treuheit, M J; Costello, C E; Kirley, T L 1993. Structures of the complex glycans found on the beta-subunit of (Na,K)-ATPase. The Journal of biological chemistry, 268 19, 13914-9

Treuheit, M J; Kirley, T L 1993. Reversibility of cysteine labeling by 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole. Analytical biochemistry, 212 1, 138-42

M.J. Treuheit, A. Ataei, T.L. Kirley and E.T. Wallick 1992. Purification of the glycosylated ß-subunit of the (Na,K)-ATPase by lectin affinity chromatography Chromatographia, 33 , 521-524

Pedemonte, C H; Kirley, T L; Treuheit, M J; Kaplan, J H 1992. Inactivation of the Na,K-ATPase by modification of Lys-501 with 4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid (SITS). FEBS letters, 314 1, 97-100

Treuheit, M J; Vaghy, P L; Kirley, T L 1992. Mg(2+)-ATPase from rabbit skeletal muscle transverse tubules is 67-kilodalton glycoprotein. The Journal of biological chemistry, 267 17, 11777-82

Cox, D A; Ellinor, P T; Kirley, T L; Matlib, M A 1991. Identification of a 17-kDa protein associated with the peripheral-type benzodiazepine receptor in vascular and other smooth muscle types. The Journal of pharmacology and experimental therapeutics, 258 2, 702-9

Kirley, T L 1991. The Mg(2+)-ATPase of rabbit skeletal-muscle transverse tubule is a highly glycosylated multiple-subunit enzyme. The Biochemical journal, 278 ( Pt 2) , 375-80

Kirley, T L; Peng, M 1991. Identification of cysteine residues in lamb kidney (Na,K)-ATPase essential for ouabain binding. The Journal of biological chemistry, 266 30, 19953-7

Hinz, H R; Kirley, T L 1990. Lysine 480 is an essential residue in the putative ATP site of lamb kidney (Na,K)-ATPase. Identification of the pyridoxal 5'-diphospho-5'-adenosine and pyridoxal phosphate reactive residue. The Journal of biological chemistry, 265 18, 10260-5

Kirley, T L 1990. Inactivation of (Na+,K+)-ATPase by beta-mercaptoethanol. Differential sensitivity to reduction of the three beta subunit disulfide bonds. The Journal of biological chemistry, 265 8, 4227-32

Kirley, T L 1989. Reduction and fluorescent labeling of cyst(e)ine-containing proteins for subsequent structural analyses. Analytical biochemistry, 180 2, 231-6

Kirley, T L 1989. Determination of three disulfide bonds and one free sulfhydryl in the beta subunit of (Na,K)-ATPase. The Journal of biological chemistry, 264 13, 7185-92

Tyson, P A; Steinberg, M; Wallick, E T; Kirley, T L 1989. Identification of the 5-iodoacetamidofluorescein reporter site on the Na,K-ATPase. The Journal of biological chemistry, 264 2, 726-34

Ball, W J; Kirley, T L; Lane, L K 1988. Preparation of antibodies to Na+,K+-ATPase and its subunits. Methods in enzymology, 156 , 87-101

Kirley, T L 1988. Purification and characterization of the Mg2+-ATPase from rabbit skeletal muscle transverse tubule. The Journal of biological chemistry, 263 25, 12682-9

Steinberg, M; Tyson, P A; Wallick, E T; Kirley, T L 1988. Identification and localization of the 5-iodoacetamido-fluorescein reporter site on dog kidney Na,K-ATPase. Progress in clinical and biological research, 273 , 39-44

Nakayama, N; Kirley, T L; Vaghy, P L; McKenna, E; Schwartz, A 1987. Purification of putative Ca2+ channel protein from rabbit skeletal muscle. Determination of the amino-terminal sequence. The Journal of biological chemistry, 262 14, 6572-6

Kirley, T L; Lane, L K; Wallick, E T 1986. Identification of an essential sulfhydryl group in the ouabain binding site of (Na,K)-ATPase. The Journal of biological chemistry, 261 10, 4525-8

Lane, L K; Kirley, T L; Ball, W J 1986. Structural studies on H+,K+-ATPase: determination of the NH2-terminal amino acid sequence and immunological cross-reactivity with Na+,K+-ATPase. Biochemical and biophysical research communications, 138 1, 185-92

Friedman, M L; Schlueter, K T; Kirley, T L; Halsall, H B 1985. Fluorescence quenching of human orosomucoid. Accessibility to drugs and small quenching agents. The Biochemical journal, 232 3, 863-7

Kirley, T L; Wang, T; Wallick, E T; Lane, L K 1985. Homology of ATP binding sites from Ca2+ and (Na,K)-ATPases: comparison of the amino acid sequences of fluorescein isothiocyanate labeled peptides. Biochemical and biophysical research communications, 130 2, 732-8

Kirley, T L; Schwartz, A 1984. Solubilization and affinity labeling of a dihydropyridine binding site from skeletal muscle: effects of temperature and diltiazem on [3H]dihydropyridine binding to transverse tubules. Biochemical and biophysical research communications, 123 1, 41-9

Kirley, T L; Wallick, E T; Lane, L K 1984. The amino acid sequence of the fluorescein isothiocyanate reactive site of lamb and rat kidney Na+- and K+-dependent ATPase. Biochemical and biophysical research communications, 125 2, 767-73

Halsall, H B; Kirley, T L 1982. The denaturation of orosomucoid. Archives of biochemistry and biophysics, 216 2, 392-9

Halsall, H B; Kirley, T L; Friedman, M L 1982. The preparation of orosomucoid from nephrotic urine. Preparative biochemistry, 12 2, 111-20

Kirley, T L; Halsall, H B 1982. Facile synthesis of spin labelled steroids by esterification. Journal of steroid biochemistry, 16 1, 133-4

Kirley, T L; Sprague, E D; Halsall, H B 1982. The binding of spin-labeled propranolol and spin-labeled progesterone by orosomucoid. Biophysical chemistry, 15 3, 209-16

Halsall, H B; Kirley, T L 1981. Observations on the thermostability of orosomucoid. Biochemical and biophysical research communications, 102 2, 761-5

Kirley T.L.;Greis K.D.;Norman A.B. 12-01-2018. Domain unfolding of monoclonal antibody fragments revealed by non-reducing SDS-PAGE Biochemistry and Biophysics Reports, 16 , 138-144

Basu S.;Murphy-Piedmonte D.;Kirley T. 12-01-2004. Conserved lysine 79 is important for activity of ecto-nucleoside triphosphate diphosphohydrolase 3 ( Purinergic Signalling, 1 1, 51-58

Chai Q.;Huang Y.;Kirley T.L.;Ayres N. 09-14-2017. Shape memory polymer foams prepared from a heparin-inspired polyurethane/urea Polymer Chemistry, 8 34, 5039-5048

Kirley T.L.;Norman A.B. 09-01-2020. Ligand binding to a humanized anti-cocaine mAb detected by non-reducing SDS-PAGE Biochemistry and Biophysics Reports, 23 ,

Basu S.;Kirley T. 09-01-2005. Identification of a tyrosine residue responsible for N- acetylimidazole-induced increase of activity Purinergic Signalling, 1 3, 271-280

Kukulski F.;Levesque S.;Lavoie E.;Lecka J.;Bigonnesse F.;Knowles A.;Robson S.;Kirley T.;Sevigny J. 09-01-2005. Erratum: Comparative hrydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8. (Purinergic Sign Purinergic Signalling, 1 3, 293

Kukulski F.;Lévesque S.;Lavoie E.;Lecka J.;Bigonnesse F.;Knowles A.;Robson S.;Kirley T.;Sévigny J. 06-01-2005. Comparative hydrolysis of P2 receptor agonists by NTPDases 1, 2, 3 and 8 Purinergic Signalling, 1 2, 193-204

Treuheit M.;Ataei A.;Kirley T.;Wallick E. 06-01-1992. Purification of the glycosylated beta subunit of (Na,K)-ATPase by lectin affinity chromatography Chromatographia, 33 11-12, 521-524

Webster R.P.;Cohen C.F.;Saeed F.O.;Wetzel H.N.;Ball W.J.;Kirley T.L.;Norman A.B. 05-01-2018. Evaluation of methods to reduce background using the Python-based ELISA_QC program Journal of Immunological Methods, 456 , 61-66

Kirley T.;Crawford P.;Smith T. 05-01-2006. The structure of the nucleoside triphosphate diphosphohydrolases (NTPDases) as revealed by mutagenic Purinergic Signalling, 2 2, 379-389

Smith T.;Kirley T. 05-01-2006. The calcium activated nucleotidases: A diverse family of soluble and membrane associated nucleotide Purinergic Signalling, 2 2, 327-333

Argekar S.;Kirley T.;Schaefer D. 04-28-2013. Determination of structure-property relationships for 3- aminopropyltriethoxysilane films using x-ra Journal of Materials Research, 28 8, 1118-1128

Gaddie K.;Kirley T. 03-29-2010. Proline residues link the active site to transmembrane domain movements in human nucleoside triphosp Purinergic Signalling, 6 3, 327-337

Murphy D.;Kirley T. 01-01-2003. Erratum: Asparagine 81, an invariant glycosylation site near apyrase conserved region 1, is essentia Archives of Biochemistry and Biophysics, 419 2, 251-252

Treuheit M.;Kirley T. 01-01-1994. Instability of Cysteine Labeling With 4-(aminosulfonyl)-7-fluoro-2, 1, 3-benzoxadiazole (ABD-F) Techniques in Protein Chemistry, 5 C, 189-194

Treuheit M.J.;Kirley T.L. 01-01-1993. Reversibility of cysteine labeling by 4-(aminosulfonyl)-7-fluoro-2,1,3-benzoxadiazole Analytical Biochemistry, 212 1, 138-142

Kirley T.;Lane L.;Wallick E. 01-01-1985. Effect of modification of tyrosine residues on (Na,K)-ATPase by tetranitromethane Federation Proceedings, 44 3,

Sprague E.;Kirley T.;Halsall H. 01-01-1982. Propranolol and progesterone binding to orosomucoid Biophysical Journal, 37 2 II,

T.L. Kirley, W.L. Seibel, and M.D. Wortman 2014. Small molecule inhibitors of Ca-activated and NTPDase classes of nucleotidases Purines 2014 international meeting, Invited Speaker, Bonn, Germany, July, 2014, ,

J. Lecka, M.M. Munkonda, M. Fausther, É.G. Lavoie, G. Kauffenstein, S.A. Levesque, I. Kogan, J. Pelletier, A. Tramblay, M.S. Rana, V.V. Ivanenkov, T.L. Kirley, B. Fischer, and J. Sévigny 2008. Specific NTPDase Inhibitors Purines 2008, Copenhagen, Denmark, ,

M.M. Munkonda, M. Fausther, V.V. Ivanenkov, J. Pelletier, A. Tremblay, B. Künzli, T.L. Kirley, and J. Sévigny 2008. Generation and characterization of a monoclonal antibody as the first specific inhibitor of human NTPDase3 Purines 2008, Copenhagen, Denmark, ,

T. L. Kirley 2006. Progress on the Structures and Functions of Nucleoside Triphosphate Diphosphohydrolases and a Calcium Activated Nucleotidase 8th International Adenosine and Adenine Nucleotides Symposium, Ferrara, Italy, May 2006, ,

T.L. Kirley, A. Zsarnovszky, P.A. Crawford, L. Spurling and S.M. Belcher 2005. Immunolocalization of ecto-nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) in rat brain: Implications for modulation of multiple homeostatic systems including feeding and sleep-wake behaviors Neuroscience Meeting 2005, Washington, D.C., November 2005, ,

T.L. Kirley 2004. Structure-function studies on nucleoside triphosphate diphosphohydrolases (NTPDases) 4th International Symposium of Nucleosides and Nucleotides (Purines 2004), Chapel Hill, N.C., ,

V.V. Ivanenkov and T.L. Kirley 2004. Characterization of disulfide bonds and inter-subunit contacts in human nucleoside triphosphate diphosphohydrolase 3 (NTPDase3) 4th International Symposium of Nucleosides and Nucleotides (Purines 2004), Chapel Hill, N.C., ,

D.M. Murphy and T.L. Kirley 2002. Site-Directed Mutagenesis of a Highly Conserved Glycosylation Site in the ACR1 region of Human Nucleoside Triphosphate Diphosphohydrolase-3 The 3rd International Workshop on Ecto-ATPases and Related Ecto-Nucleotidases, Woods Hole, MA, ,

D.M. Murphy, V.V. Ivanenkov and T.L. Kirley 2002. Structure-Function of Nucleotide Triphosphate Diphosphohydrolases: Mutagenesis of Membranous NTPDase3 and Bacterial Expression and Characterization of Soluble NTPDase6 The 3rd International Workshop on Ecto-ATPases and Related Ecto-Nucleotidases, Woods Hole, MA, ,

D.M. Murphy, V.V. Ivanenkov, F. Yang, C.A. Hicks-Berger, T.M. Smith and T.L. Kirley 2002. Site-directed mutagenesis of cell membrane NTPDases: Implications for structure and function of the ecto-apyrases and ecto-ATPases The 7th International Symposium on Adenosine & Adenine Nucleotides, Gold Coast, Australia, ,

Sevigny J., JA. Dranoff, N. Braun, R. Lemmens, E. Kruglov, E. Csizmadia, R.N. Smith, H. Zimmermann, T.L. Kirley and S.C. Robson 2001. Identification and characterization of membrane-associated hepatic NTP diphosphohydrolases. 52nd Annual Meeting of the American Association for the Study of Liver Diseases (AASLD), Dallas, TX, USA. Hepathology, 34 , 451A

C. Hicks-Berger, B. P. Chadwick, A-M Frischauf and T. L. Kirley 2000. Expression and Initial Characterization of CD39L2 Ecto-nucleotidase: a Soluble GDPase ASBMB/ASPET meeting, Boston MA, ,

T. M. Smith and T.L. Kirley 1999. Site-directed mutagenesis of an E-type ATPase: Relatedness to the actin/HSP 70/sugar kinase superfamily and conversion of an ecto-apyrase to an ecto-NTPase Second International Workshop on Ecto-ATPases and Related Ectonucleotidases, ,

T.L. Kirley and T. M. Smith 1999. cDNA cloning, sequencing, and expression of an ecto-apyrase from human brain: Importance of glycosylation and oligomerization Second International Workshop on Ecto-ATPases and Related Ectonucleotidases, ,

T.L. Kirley and J. G. Stout 1996. Fragmentation, cDNA Cloning and Sequencing of the Chicken Muscle ecto-ATPase First International Workshop on Ecto-ATPases, ,

T.L. Kirley and J. G. Stout 1996. Ecto-ATPase Activity is Controlled by Oligomerization State First International Workshop on Ecto-ATPases, ,

T.L. Kirley, R. S. Strobel and J. G. Stout 1996. Purification and Characterization of the Chicken Gizzard (smooth muscle) ecto-ATPase First International Workshop on Ecto-ATPases, ,

T.M. Smith, T.L. Kirley and T.M. Hennessey 1996. Purification and Characterization of a Soluble Ecto-ATPase from Tetrahymena Thermophilia First International Workshop on Ecto-ATPases, ,

J. G. Stout, A. Brittsan and T.L. Kirley 1994. Brain Mg-ATPase is Not the Neural Cell Adhesion Molecule Protein Society Meeting, ,

J.G. Stout and T.L. Kirley 1994. Characterization of Chicken Muscle Ecto-Mg-ATPases Biophys. J., 66 , A118

M. Peng, R. Diebold, H. Fan, A. Caswell, T.L. Kirley, J. Wang and A. Schwartz 1994. Isolation and Tissue Distribution of Three Triadin Isoforms in Rabbit Skeletal Muscle Biophys. J., 66 ,

E.T.Wallick, T.L.Kirley, M.J.Treuheit and A. Ataei 1993. Purification and characterization of the alpha and beta subunits of Na,K-ATPase Protein Society Meeting, ,

M.J. Treuheit and T.L. Kirley 1993. Reversibility of Cysteine Labeling by 4-(Aminosulfonyl)-7-Fluoro-2,1,3-Benzoxadiazole (ABD-F) Protein Society Meeting, ,

M.J. Treuheit, C.E. Costello and T.L. Kirley 1993. Structures of the complex glycans found on the ß-subunits of lamb and dog kidney (Na,K)-ATPase Biophys. J., 64 , A371

T.L. Kirley 1993. Purification of Mg-ATPase of Chicken Gizzard and Heart - Comparison to the Rabbit Skeletal Enzyme Protein Society Meeting, ,

C.H. Pedemonte, J.H. Kaplan, M.J. Treuheit and T.L. Kirley 1992. Inactivation and the labeling of the (Na,K)-ATPase by SITS and H2-DIDS Biophys. J., 61 , A121 (702)

M.J. Treuheit and T.L. Kirley 1992. The transverse tubule Mg-ATPase is a 70 kDa glycoprotein Biophys. J., 61 , A117 (675)

T.L. Kirley and M.J. Treuheit 1992. Purification of rabbit skeletal muscle transverse tubule magnesium ATPase for structural analyses Sixth Symposium of the Protein Society, T87 ,

M. Treuheit and T.L. Kirley 1991. Structures of the complex glycans found in the ß-subunit of Na,K-ATPase Glycoconjugate J., 8 , 258-259

T.L. Kirley 1991. The transverse tubule Mg-ATPase is a highly glycosylated multiple subunit enzyme Biophys. J., 59 , 113a

D.A. Cox, P.T. Ellinor, W. Koch, T. Kirley and M.A. Matlib 1990. Identification of the peripheral-type benzodiazepine binding site (PBS) in vascular smooth muscle ASPET, ,

G. Kelso, T. L. Kirley and J.A.K. Harmony 1990. Analysis of disulfide bonds within the novel Apolipoprotein J. isolated from human plasma Fourth Symposium of the Protein Society, San Diego, CA, ,

H.R. Hinz and T.L. Kirley 1990. Identification of a reactive lys residue in the putative ATP binding site of lamb kidney (Na,K)-ATPase Biophys. J., 57 , 356a

T.L. Kirley 1990. Examination of possible subunit structure of the transverse tubule Mg-ATPase Biophys. J., 57 , 38a

E. McKenna, P.L. Vaghy, K. Naito, K. Itagaki, T.L. Kirley and A. Schwartz 1989. Analysis of the putative subunits of a voltage-dependent calcium channel Biophys. J., 55 , 301a

T.L. Kirley 1988. Purification and characterization of the Mg-ATPase from rabbit skeletal muscle Biophys. J., 53 , 486a

T.L. Kirley 1988. Identification of three disulfide bonds and one free sulfhdryl in the ß subunit of (Na,K)-ATPase Biophys. J., 55 , 425a

T.L. Kirley 1986. Inhibition of the rabbit skeletal muscle transverse tubule Mg-ATPase by diethylpyrocarbonate Biophys. J., 49 , 554a

T.L. Kirley, E.T. Wallick, T. Wang and L.K. Lane 1985. Species and tissue comparisons of the amino acid sequence of the ATP binding sites of (Na,K)-ATPase and Ca-ATPase Biophys. J., 47 , 182a

T.L. Kirley, L.K. Lane and E.T. Wallick 1985. Effect of modification of tyrosine residues on (Na,K)-ATPase by tetranitromethane Fed. Proc., 44 , 1172

T.L. Kirley and A. Schwartz 1984. 3H-Nimodipine and 3H-nitrendipine binding to rabbit skeletal t-tubular membranes: effects of d-cis-diltiazem and attempts to solubilize and affinity label the putative calcium channel Biophys. J., 45 , 396a

H.B. Halsall, T.L. Kirley, J.A. Wallace, E.D. Sprague and M.L. Friedman 1983. Steroid and drug binding to human orosomucoid Fed. Proc., 42 , 2102

E.D. Sprague, T.L. Kirley and H.B. Halsall 1982. Propranolol and progesterone binding to orosomucoid Biophys. J., 37 , 383a

T.L. Kirley and H.B. Halsall 1982. The thermal stability of orosomucoid Biophys J., 37 , 390a

H.B. Halsall, T.L. Kirley and E.D. Sprague 1980. Preparation and binding of spin labeled steroids to orosomucoid Fed. Proc., 39 , 1906

T.L. Kirley . An improved method for electroblotting of protein from SDS-PAGE gels for direct microsequencing Proc. of the Sixth International Conference on Methods in Protein Sequence Analysis, ,