Glucose Metabolism
Glucose Metabolism
C1070-Intraperitoneal glucose tolerance test (IP-GTT)
Mice will be fasted for 5 hours. A fasting blood sample will be removed from the tail vein and a concentrated solution of glucose injected into the abdominal cavity of the mice through a needle passed through the abdominal skin.. Blood samples will be removed from the tail vein 15, 30, 60 and 120 minutes later. Samples of plasma obtained during the test will be measured for concentrations of glucose and insulin.
C 1071-Oral glucose tolerance test (O-GTT)
Mice will be fasted for 5 hours. A fasting blood sample will be removed from the tail vein and a concentrated solution of glucose orally gavaged into the stomach of the mice. Blood samples will be removed from the tail vein 15, 30, 60 and 120 minutes later. Samples of plasma obtained during the test will be measured for concentrations of glucose and insulin.
C1072-Insulin tolerance test (ITT)
Mice will be fasted for 5 hours. A fasting blood sample will be removed from the tail vein and insulin (0.5mU/g) will be injected into the abdominal cavity. Blood samples will be removed from the tail vein 15, 30, 45 and 60 minutes later. Samples of plasma obtained during the test will be measured for concentrations of glucose and insulin.
C1085-Glucagon concentration
Concentrations of glucagon in plasma or serum are determined using a multiplexing kit available from Millipore Inc. RIA kits are also available from Millipore for measurement of glucagon.
C1087-Glucose concentration
Determinations of glucose in plasma/serum/lymph will be made using a specific colorimetric assay. Reactions will be run in microtiter plates and analyzed on a plate reader. Using this method assays can be performed on small (5-25ul) samples of plasma or serum.
C1089-Insulin Concentration
Concentrations of insulin in plasma or serum are determined using a multiplexing kit available from Millipore Inc. ELISA kits are also available from Millipore for measurement of insulin.
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UC Reading Campus
Metabolic Diseases Institute
2120 E. Galbraith Rd.
Cincinnati, OH 45237
Phone: 513-558-2748