Education/CredentialsPostdoctoral Fellowship : Northwestern University (Neurobiology)Doctoral Degree: University of Wisconsin (Biochemistry)Bachelor's Degree: University of Michigan (Organic Chemistry) Contact Information Email kleenesj@ucmail.uc.edu Research InterestsThe Kleene lab studies signaling in primary cilia as it relates to kidney disease. Primary cilia are present on most mammalian cells. They influence fundamental cellular processes, and defects in primary cilia contribute to kidney disease, cancer, and obesity. Our work focuses on polycystic kidney disease (PKD), a renal disease that is often caused by defects in ion-conducting channels in the primary cilia. To that end, we have developed... Peer Reviewed Publications Kleene, Steven J 2022. Regenerative calcium currents in renal primary cilia Frontiers in Physiology, 13 , 894518Kleene, Steven J 2022. Regenerative Calcium Currents in Renal Primary Cilia. Frontiers in physiology, 13 , 894518Kleene, Steven J; Kleene, Nancy K 2021. Inward calcium current through the polycystin-2-dependent channels of renal primary cilia American Journal of Physiology Renal Physiology, 320 , F1165Kleene, Steven J; Kleene, Nancy K 2021. Inward Ca2+ current through the polycystin-2-dependent channels of renal primary cilia. American journal of physiology. Renal physiology, 320 6, F1165-F1173Kleene, Steven J; Siroky, Brian J; Landero-Figueroa, Julio A; Dixon, Bradley P; Pachciarz, Nolan W; Lu, Lu; Kleene, Nancy K 2019. The TRPP2-dependent channel of renal primary cilia also requires TRPM3. PloS one, 14 3, e0214053Kleene, Steven J; Kleene, Nancy K 2017. The native TRPP2-dependent channel of murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F96Siroky, Brian J; Kleene, Nancy K; Kleene, Steven J; Varnell, Charles D; Comer, Raven G; Liu, Jialiu; Lu, Lu; Pachciarz, Nolan W; Bissler, John J; Dixon, Bradley P 2017. Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3 American Journal of Physiology Renal Physiology, 312 , F791Nauli, Surya M; Pala, Rajasekharreddy; Kleene, Steven J 2016. Calcium channels in primary cilia Current Opinion in Nephrology and Hypertension, 25 , 452Flannery, Richard J; Kleene, Nancy K; Kleene, Steven J 2015. A TRPM4-dependent current in murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F697Kleene, Steven J; Van Houten, Judith L 2014. Electrical Signaling in Motile and Primary Cilia Bioscience, 64 , 1092-1102Griff, Edwin R; Kleene, Nancy K; Kleene, Steven J 2012. A selective PMCA inhibitor does not prolong the electroolfactogram in mouse PLoS ONE, 7 , e37148Kleene, Nancy K; Kleene, Steven J 2012. A method for measuring electrical signals in a primary cilium Cilia, 1 , 17French, Donald A; Badamdorj, Dorjsuren; Kleene, Steven J 2010. Spatial distribution of calcium-gated chloride channels in olfactory cilia PLoS ONE, 5 , e15676Kleene, Steven J 2009. Identifying olfaction's 'other channels' The Journal of Physiology, 587 , 4135-4136Kleene, Steven J 2009. Limits of calcium clearance by plasma membrane calcium ATPase in olfactory cilia PLoS ONE, 4 , e5266Badamdorj, Dorjsuren; Edwards, David A; French, Donald A; Kleene, Steven J 2008. Identification of Cl(Ca) channel distributions in olfactory cilia Mathematical Methods in the Applied Sciences, 31 , 1860-1873Kleene, Steven J 2008. The electrochemical basis of odor transduction in vertebrate olfactory cilia Chemical Senses, 33 , 839-859Smith, David W; Thach, Sokunthirith; Marshall, Erika L; Mendoza, Mary-Grace; Kleene, Steven J 2008. Mice lacking NKCC1 have normal olfactory sensitivity Physiology & Behavior, 93 , 44-49Nickell, William T; Kleene, Nancy K; Kleene, Steven J 2007. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium The Journal of Physiology, 583 , 1005-1020Flannery, Richard J; French, Donald A; Kleene, Steven J 2006. Clustering of cyclic-nucleotide-gated channels in olfactory cilia Biophysical Journal, 91 , 179-188French, Donald A; Flannery, Richard J; Groetsch, Charles W; Krantz, Willam B; Kleene, Steven J 2006. Numerical approximation of solutions of a nonlinear inverse problem arising in olfaction experimentation Mathematical and Computer Modelling, 43 7-8, 945-956Nickell, William T; Kleene, Nancy K; Gesteland, Robert C; Kleene, Steven J 2006. Neuronal chloride accumulation in olfactory epithelium of mice lacking NKCC1 Journal of Neurophysiology, 95 , 2003-2006Pun, Raymund Y K; Kleene, Steven J 2004. An estimate of the resting membrane resistance of frog olfactory receptor neurones The Journal of Physiology, 559 , 535-542Pun, Raymund Y K; Kleene, Steven J 2003. Contribution of cyclic-nucleotide-gated channels to the resting conductance of olfactory receptor neurons Biophysical Journal, 84 , 3425-3435Nickell, W T; Pun, R Y K; Bargmann, C I; Kleene, S J 2002. Single ionic channels of two Caenorhabditis elegans chemosensory neurons in native membrane The Journal of Membrane Biology, 189 , 55-66Pun, Raymund Y K; Kleene, Steven J 2002. Outward currents in olfactory receptor neurons activated by odorants and by elevation of cyclic AMP Cell Biochemistry and Biophysics, 37 , 15-26Frings, S; Reuter, D; Kleene, S J 2000. Neuronal Ca2+ -activated Cl- channels--homing in on an elusive channel species Progress in Neurobiology, 60 , 247-289Kleene, S J 2000. Spontaneous gating of olfactory cyclic-nucleotide-gated channels The Journal of Membrane Biology, 178 , 49-54Kleene, S J 1999. Both external and internal calcium reduce the sensitivity of the olfactory cyclic-nucleotide-gated channel to cAMP Journal of Neurophysiology, 81 , 2675-2682Kleene, S J 1997. High-gain, low-noise amplification in olfactory transduction Biophysical Journal, 73 , 1110-1117Larsson, H P; Kleene, S J; Lecar, H 1997. Noise analysis of ion channels in non-space-clamped cables: estimates of channel parameters in olfactory cilia Biophysical Journal, 72 , 1193-1203Kleene, S J; Pun, R Y 1996. Persistence of the olfactory receptor current in a wide variety of extracellular environments Journal of Neurophysiology, 75 , 1386-1391Kleene, S J 1995. Block by external calcium and magnesium of the cyclic-nucleotide-activated current in olfactory cilia Neuroscience, 66 , 1001-1008Kleene, S J 1994. Inhibition of olfactory cyclic nucleotide-activated current by calmodulin antagonists British Journal of Pharmacology, 111 , 469-472Kleene, S J; Cejtin, H C 1994. Solving buffering problems with Mathematica software Analytical Biochemistry, 222 , 310-314Kleene, S J; Gesteland, R C; Bryant, S H 1994. An electrophysiological survey of frog olfactory cilia The Journal of Experimental Biology, 195 , 307-328Pun, R Y; Kleene, S J; Gesteland, R C 1994. Guanine nucleotides modulate steady-state inactivation of voltage-gated sodium channels in frog olfactory receptor neurons The Journal of Membrane Biology, 142 , 103-111Kleene, S J 1993. Origin of the chloride current in olfactory transduction Neuron, 11 , 123-132Kleene, S J 1993. The cyclic nucleotide-activated conductance in olfactory cilia: effects of cytoplasmic Mg2+ and Ca2+ The Journal of Membrane Biology, 131 , 237-243Kleene, S J 1993. A simple intrapipette salt bridge Journal of Neuroscience Methods, 46 , 11-16Kleene, S J 1992. Basal conductance of frog olfactory cilia Pflügers Archiv : European Journal of Physiology, 421 , 374-380Kleene, S J; Gesteland, R C 1991. Calcium-activated chloride conductance in frog olfactory cilia The Journal of Neuroscience, 11 , 3624-3629Kleene, S J; Gesteland, R C 1991. Transmembrane currents in frog olfactory cilia The Journal of Membrane Biology, 120 , 75-81Lidow, M S; Gesteland, R C; Shipley, M T; Kleene, S J 1987. Comparative study of immature and mature olfactory receptor cells in adult frogs Developmental Brain Research, 31 , 243-258Kleene, S J 1986. Bacterial chemotaxis and vertebrate olfaction Experientia, 42 , 241-250Lidow, M S; Kleene, S J; Gesteland, R C 1986. Suppression of mitotic activity and synchronization of cell development in olfactory epithelium Developmental Brain Research, 28 , 145-162Kleene, S J; Gesteland, R C 1983. Dissociation of frog olfactory epithelium Journal of Neuroscience Methods, 9 , 173-183Kleene, S J; Gesteland, R C 1981. Dissociation of frog olfactory epithelium with N-ethylmaleimide Brain Research, 229 , 536-540Kleene, S J; Hobson, A C; Adler, J 1979. Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli Proceedings of the National Academy of Sciences of the United States of America, 76 , 6309-6313Kleene, S J; Toews, M L; Adler, J 1977. Isolation of glutamic acid methyl ester from an Escherichia coli membrane protein involved in chemotaxis The Journal of Biological Chemistry, 252 , 3214-3218
Research InterestsThe Kleene lab studies signaling in primary cilia as it relates to kidney disease. Primary cilia are present on most mammalian cells. They influence fundamental cellular processes, and defects in primary cilia contribute to kidney disease, cancer, and obesity. Our work focuses on polycystic kidney disease (PKD), a renal disease that is often caused by defects in ion-conducting channels in the primary cilia. To that end, we have developed...
Peer Reviewed Publications Kleene, Steven J 2022. Regenerative calcium currents in renal primary cilia Frontiers in Physiology, 13 , 894518Kleene, Steven J 2022. Regenerative Calcium Currents in Renal Primary Cilia. Frontiers in physiology, 13 , 894518Kleene, Steven J; Kleene, Nancy K 2021. Inward calcium current through the polycystin-2-dependent channels of renal primary cilia American Journal of Physiology Renal Physiology, 320 , F1165Kleene, Steven J; Kleene, Nancy K 2021. Inward Ca2+ current through the polycystin-2-dependent channels of renal primary cilia. American journal of physiology. Renal physiology, 320 6, F1165-F1173Kleene, Steven J; Siroky, Brian J; Landero-Figueroa, Julio A; Dixon, Bradley P; Pachciarz, Nolan W; Lu, Lu; Kleene, Nancy K 2019. The TRPP2-dependent channel of renal primary cilia also requires TRPM3. PloS one, 14 3, e0214053Kleene, Steven J; Kleene, Nancy K 2017. The native TRPP2-dependent channel of murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F96Siroky, Brian J; Kleene, Nancy K; Kleene, Steven J; Varnell, Charles D; Comer, Raven G; Liu, Jialiu; Lu, Lu; Pachciarz, Nolan W; Bissler, John J; Dixon, Bradley P 2017. Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3 American Journal of Physiology Renal Physiology, 312 , F791Nauli, Surya M; Pala, Rajasekharreddy; Kleene, Steven J 2016. Calcium channels in primary cilia Current Opinion in Nephrology and Hypertension, 25 , 452Flannery, Richard J; Kleene, Nancy K; Kleene, Steven J 2015. A TRPM4-dependent current in murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F697Kleene, Steven J; Van Houten, Judith L 2014. Electrical Signaling in Motile and Primary Cilia Bioscience, 64 , 1092-1102Griff, Edwin R; Kleene, Nancy K; Kleene, Steven J 2012. A selective PMCA inhibitor does not prolong the electroolfactogram in mouse PLoS ONE, 7 , e37148Kleene, Nancy K; Kleene, Steven J 2012. A method for measuring electrical signals in a primary cilium Cilia, 1 , 17French, Donald A; Badamdorj, Dorjsuren; Kleene, Steven J 2010. Spatial distribution of calcium-gated chloride channels in olfactory cilia PLoS ONE, 5 , e15676Kleene, Steven J 2009. Identifying olfaction's 'other channels' The Journal of Physiology, 587 , 4135-4136Kleene, Steven J 2009. Limits of calcium clearance by plasma membrane calcium ATPase in olfactory cilia PLoS ONE, 4 , e5266Badamdorj, Dorjsuren; Edwards, David A; French, Donald A; Kleene, Steven J 2008. Identification of Cl(Ca) channel distributions in olfactory cilia Mathematical Methods in the Applied Sciences, 31 , 1860-1873Kleene, Steven J 2008. The electrochemical basis of odor transduction in vertebrate olfactory cilia Chemical Senses, 33 , 839-859Smith, David W; Thach, Sokunthirith; Marshall, Erika L; Mendoza, Mary-Grace; Kleene, Steven J 2008. Mice lacking NKCC1 have normal olfactory sensitivity Physiology & Behavior, 93 , 44-49Nickell, William T; Kleene, Nancy K; Kleene, Steven J 2007. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium The Journal of Physiology, 583 , 1005-1020Flannery, Richard J; French, Donald A; Kleene, Steven J 2006. Clustering of cyclic-nucleotide-gated channels in olfactory cilia Biophysical Journal, 91 , 179-188French, Donald A; Flannery, Richard J; Groetsch, Charles W; Krantz, Willam B; Kleene, Steven J 2006. Numerical approximation of solutions of a nonlinear inverse problem arising in olfaction experimentation Mathematical and Computer Modelling, 43 7-8, 945-956Nickell, William T; Kleene, Nancy K; Gesteland, Robert C; Kleene, Steven J 2006. Neuronal chloride accumulation in olfactory epithelium of mice lacking NKCC1 Journal of Neurophysiology, 95 , 2003-2006Pun, Raymund Y K; Kleene, Steven J 2004. An estimate of the resting membrane resistance of frog olfactory receptor neurones The Journal of Physiology, 559 , 535-542Pun, Raymund Y K; Kleene, Steven J 2003. Contribution of cyclic-nucleotide-gated channels to the resting conductance of olfactory receptor neurons Biophysical Journal, 84 , 3425-3435Nickell, W T; Pun, R Y K; Bargmann, C I; Kleene, S J 2002. Single ionic channels of two Caenorhabditis elegans chemosensory neurons in native membrane The Journal of Membrane Biology, 189 , 55-66Pun, Raymund Y K; Kleene, Steven J 2002. Outward currents in olfactory receptor neurons activated by odorants and by elevation of cyclic AMP Cell Biochemistry and Biophysics, 37 , 15-26Frings, S; Reuter, D; Kleene, S J 2000. Neuronal Ca2+ -activated Cl- channels--homing in on an elusive channel species Progress in Neurobiology, 60 , 247-289Kleene, S J 2000. Spontaneous gating of olfactory cyclic-nucleotide-gated channels The Journal of Membrane Biology, 178 , 49-54Kleene, S J 1999. Both external and internal calcium reduce the sensitivity of the olfactory cyclic-nucleotide-gated channel to cAMP Journal of Neurophysiology, 81 , 2675-2682Kleene, S J 1997. High-gain, low-noise amplification in olfactory transduction Biophysical Journal, 73 , 1110-1117Larsson, H P; Kleene, S J; Lecar, H 1997. Noise analysis of ion channels in non-space-clamped cables: estimates of channel parameters in olfactory cilia Biophysical Journal, 72 , 1193-1203Kleene, S J; Pun, R Y 1996. Persistence of the olfactory receptor current in a wide variety of extracellular environments Journal of Neurophysiology, 75 , 1386-1391Kleene, S J 1995. Block by external calcium and magnesium of the cyclic-nucleotide-activated current in olfactory cilia Neuroscience, 66 , 1001-1008Kleene, S J 1994. Inhibition of olfactory cyclic nucleotide-activated current by calmodulin antagonists British Journal of Pharmacology, 111 , 469-472Kleene, S J; Cejtin, H C 1994. Solving buffering problems with Mathematica software Analytical Biochemistry, 222 , 310-314Kleene, S J; Gesteland, R C; Bryant, S H 1994. An electrophysiological survey of frog olfactory cilia The Journal of Experimental Biology, 195 , 307-328Pun, R Y; Kleene, S J; Gesteland, R C 1994. Guanine nucleotides modulate steady-state inactivation of voltage-gated sodium channels in frog olfactory receptor neurons The Journal of Membrane Biology, 142 , 103-111Kleene, S J 1993. Origin of the chloride current in olfactory transduction Neuron, 11 , 123-132Kleene, S J 1993. The cyclic nucleotide-activated conductance in olfactory cilia: effects of cytoplasmic Mg2+ and Ca2+ The Journal of Membrane Biology, 131 , 237-243Kleene, S J 1993. A simple intrapipette salt bridge Journal of Neuroscience Methods, 46 , 11-16Kleene, S J 1992. Basal conductance of frog olfactory cilia Pflügers Archiv : European Journal of Physiology, 421 , 374-380Kleene, S J; Gesteland, R C 1991. Calcium-activated chloride conductance in frog olfactory cilia The Journal of Neuroscience, 11 , 3624-3629Kleene, S J; Gesteland, R C 1991. Transmembrane currents in frog olfactory cilia The Journal of Membrane Biology, 120 , 75-81Lidow, M S; Gesteland, R C; Shipley, M T; Kleene, S J 1987. Comparative study of immature and mature olfactory receptor cells in adult frogs Developmental Brain Research, 31 , 243-258Kleene, S J 1986. Bacterial chemotaxis and vertebrate olfaction Experientia, 42 , 241-250Lidow, M S; Kleene, S J; Gesteland, R C 1986. Suppression of mitotic activity and synchronization of cell development in olfactory epithelium Developmental Brain Research, 28 , 145-162Kleene, S J; Gesteland, R C 1983. Dissociation of frog olfactory epithelium Journal of Neuroscience Methods, 9 , 173-183Kleene, S J; Gesteland, R C 1981. Dissociation of frog olfactory epithelium with N-ethylmaleimide Brain Research, 229 , 536-540Kleene, S J; Hobson, A C; Adler, J 1979. Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli Proceedings of the National Academy of Sciences of the United States of America, 76 , 6309-6313Kleene, S J; Toews, M L; Adler, J 1977. Isolation of glutamic acid methyl ester from an Escherichia coli membrane protein involved in chemotaxis The Journal of Biological Chemistry, 252 , 3214-3218
Kleene, Steven J 2022. Regenerative calcium currents in renal primary cilia Frontiers in Physiology, 13 , 894518Kleene, Steven J 2022. Regenerative Calcium Currents in Renal Primary Cilia. Frontiers in physiology, 13 , 894518Kleene, Steven J; Kleene, Nancy K 2021. Inward calcium current through the polycystin-2-dependent channels of renal primary cilia American Journal of Physiology Renal Physiology, 320 , F1165Kleene, Steven J; Kleene, Nancy K 2021. Inward Ca2+ current through the polycystin-2-dependent channels of renal primary cilia. American journal of physiology. Renal physiology, 320 6, F1165-F1173Kleene, Steven J; Siroky, Brian J; Landero-Figueroa, Julio A; Dixon, Bradley P; Pachciarz, Nolan W; Lu, Lu; Kleene, Nancy K 2019. The TRPP2-dependent channel of renal primary cilia also requires TRPM3. PloS one, 14 3, e0214053Kleene, Steven J; Kleene, Nancy K 2017. The native TRPP2-dependent channel of murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F96Siroky, Brian J; Kleene, Nancy K; Kleene, Steven J; Varnell, Charles D; Comer, Raven G; Liu, Jialiu; Lu, Lu; Pachciarz, Nolan W; Bissler, John J; Dixon, Bradley P 2017. Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3 American Journal of Physiology Renal Physiology, 312 , F791Nauli, Surya M; Pala, Rajasekharreddy; Kleene, Steven J 2016. Calcium channels in primary cilia Current Opinion in Nephrology and Hypertension, 25 , 452Flannery, Richard J; Kleene, Nancy K; Kleene, Steven J 2015. A TRPM4-dependent current in murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F697Kleene, Steven J; Van Houten, Judith L 2014. Electrical Signaling in Motile and Primary Cilia Bioscience, 64 , 1092-1102Griff, Edwin R; Kleene, Nancy K; Kleene, Steven J 2012. A selective PMCA inhibitor does not prolong the electroolfactogram in mouse PLoS ONE, 7 , e37148Kleene, Nancy K; Kleene, Steven J 2012. A method for measuring electrical signals in a primary cilium Cilia, 1 , 17French, Donald A; Badamdorj, Dorjsuren; Kleene, Steven J 2010. Spatial distribution of calcium-gated chloride channels in olfactory cilia PLoS ONE, 5 , e15676Kleene, Steven J 2009. Identifying olfaction's 'other channels' The Journal of Physiology, 587 , 4135-4136Kleene, Steven J 2009. Limits of calcium clearance by plasma membrane calcium ATPase in olfactory cilia PLoS ONE, 4 , e5266Badamdorj, Dorjsuren; Edwards, David A; French, Donald A; Kleene, Steven J 2008. Identification of Cl(Ca) channel distributions in olfactory cilia Mathematical Methods in the Applied Sciences, 31 , 1860-1873Kleene, Steven J 2008. The electrochemical basis of odor transduction in vertebrate olfactory cilia Chemical Senses, 33 , 839-859Smith, David W; Thach, Sokunthirith; Marshall, Erika L; Mendoza, Mary-Grace; Kleene, Steven J 2008. Mice lacking NKCC1 have normal olfactory sensitivity Physiology & Behavior, 93 , 44-49Nickell, William T; Kleene, Nancy K; Kleene, Steven J 2007. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium The Journal of Physiology, 583 , 1005-1020Flannery, Richard J; French, Donald A; Kleene, Steven J 2006. Clustering of cyclic-nucleotide-gated channels in olfactory cilia Biophysical Journal, 91 , 179-188French, Donald A; Flannery, Richard J; Groetsch, Charles W; Krantz, Willam B; Kleene, Steven J 2006. Numerical approximation of solutions of a nonlinear inverse problem arising in olfaction experimentation Mathematical and Computer Modelling, 43 7-8, 945-956Nickell, William T; Kleene, Nancy K; Gesteland, Robert C; Kleene, Steven J 2006. Neuronal chloride accumulation in olfactory epithelium of mice lacking NKCC1 Journal of Neurophysiology, 95 , 2003-2006Pun, Raymund Y K; Kleene, Steven J 2004. An estimate of the resting membrane resistance of frog olfactory receptor neurones The Journal of Physiology, 559 , 535-542Pun, Raymund Y K; Kleene, Steven J 2003. Contribution of cyclic-nucleotide-gated channels to the resting conductance of olfactory receptor neurons Biophysical Journal, 84 , 3425-3435Nickell, W T; Pun, R Y K; Bargmann, C I; Kleene, S J 2002. Single ionic channels of two Caenorhabditis elegans chemosensory neurons in native membrane The Journal of Membrane Biology, 189 , 55-66Pun, Raymund Y K; Kleene, Steven J 2002. Outward currents in olfactory receptor neurons activated by odorants and by elevation of cyclic AMP Cell Biochemistry and Biophysics, 37 , 15-26Frings, S; Reuter, D; Kleene, S J 2000. Neuronal Ca2+ -activated Cl- channels--homing in on an elusive channel species Progress in Neurobiology, 60 , 247-289Kleene, S J 2000. Spontaneous gating of olfactory cyclic-nucleotide-gated channels The Journal of Membrane Biology, 178 , 49-54Kleene, S J 1999. Both external and internal calcium reduce the sensitivity of the olfactory cyclic-nucleotide-gated channel to cAMP Journal of Neurophysiology, 81 , 2675-2682Kleene, S J 1997. High-gain, low-noise amplification in olfactory transduction Biophysical Journal, 73 , 1110-1117Larsson, H P; Kleene, S J; Lecar, H 1997. Noise analysis of ion channels in non-space-clamped cables: estimates of channel parameters in olfactory cilia Biophysical Journal, 72 , 1193-1203Kleene, S J; Pun, R Y 1996. Persistence of the olfactory receptor current in a wide variety of extracellular environments Journal of Neurophysiology, 75 , 1386-1391Kleene, S J 1995. Block by external calcium and magnesium of the cyclic-nucleotide-activated current in olfactory cilia Neuroscience, 66 , 1001-1008Kleene, S J 1994. Inhibition of olfactory cyclic nucleotide-activated current by calmodulin antagonists British Journal of Pharmacology, 111 , 469-472Kleene, S J; Cejtin, H C 1994. Solving buffering problems with Mathematica software Analytical Biochemistry, 222 , 310-314Kleene, S J; Gesteland, R C; Bryant, S H 1994. An electrophysiological survey of frog olfactory cilia The Journal of Experimental Biology, 195 , 307-328Pun, R Y; Kleene, S J; Gesteland, R C 1994. Guanine nucleotides modulate steady-state inactivation of voltage-gated sodium channels in frog olfactory receptor neurons The Journal of Membrane Biology, 142 , 103-111Kleene, S J 1993. Origin of the chloride current in olfactory transduction Neuron, 11 , 123-132Kleene, S J 1993. The cyclic nucleotide-activated conductance in olfactory cilia: effects of cytoplasmic Mg2+ and Ca2+ The Journal of Membrane Biology, 131 , 237-243Kleene, S J 1993. A simple intrapipette salt bridge Journal of Neuroscience Methods, 46 , 11-16Kleene, S J 1992. Basal conductance of frog olfactory cilia Pflügers Archiv : European Journal of Physiology, 421 , 374-380Kleene, S J; Gesteland, R C 1991. Calcium-activated chloride conductance in frog olfactory cilia The Journal of Neuroscience, 11 , 3624-3629Kleene, S J; Gesteland, R C 1991. Transmembrane currents in frog olfactory cilia The Journal of Membrane Biology, 120 , 75-81Lidow, M S; Gesteland, R C; Shipley, M T; Kleene, S J 1987. Comparative study of immature and mature olfactory receptor cells in adult frogs Developmental Brain Research, 31 , 243-258Kleene, S J 1986. Bacterial chemotaxis and vertebrate olfaction Experientia, 42 , 241-250Lidow, M S; Kleene, S J; Gesteland, R C 1986. Suppression of mitotic activity and synchronization of cell development in olfactory epithelium Developmental Brain Research, 28 , 145-162Kleene, S J; Gesteland, R C 1983. Dissociation of frog olfactory epithelium Journal of Neuroscience Methods, 9 , 173-183Kleene, S J; Gesteland, R C 1981. Dissociation of frog olfactory epithelium with N-ethylmaleimide Brain Research, 229 , 536-540Kleene, S J; Hobson, A C; Adler, J 1979. Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli Proceedings of the National Academy of Sciences of the United States of America, 76 , 6309-6313Kleene, S J; Toews, M L; Adler, J 1977. Isolation of glutamic acid methyl ester from an Escherichia coli membrane protein involved in chemotaxis The Journal of Biological Chemistry, 252 , 3214-3218